Chemical constituents from root bark of Litsea glutinosa / 中草药

Objective: To study the chemical constituents from the root bark of Litsea glutinosa. Methods: Silica gel and Sephedex LH-20 column chromatographies as well as semi-preparative HPLC were applied to isolate and purify the compounds. Their structures were elucidated based on the spectrum analysis. Results: Fifteen compounds were obtained and identi-fied as schizandriside (1), lyonside (2), coclaurine (3), alangisesquin A (4), alangisesquin B (5), dihydrobuddlenol (6), ssioriside (7), dendranthemoside B (8), epi-anhydrocinnzeylanol (9), isolariciresinol-5’-methoxy-iso-larixa-9’-O-β-D-xylopyranoside (10), benzyl alcohol-β-D- glucopyranoside (11), phenylethyl-β-D-glucopyranoside (12), n-butyl-β-D-fructoside (13), N-cis-feruloyl tyramine (14), and N-trans-sphingoyl tyramine (15). Conclusion: Compounds 1-13 are isolated from this plant for the first time.

Research progress in Litsea glutinosa / 中草药

Litsea glutinosa, belonging to family Lauraceae, is widely distributed in subtropical and tropical regions. L. glutinosa was used to cure many diseases as folk medicine in China. It has proved that the plant showed anti-diabetic, anti-inflammatory, and anti-bacterial activities. So far, more than 50 compounds including flavonoids, alkaloids, and lignans have been isolated from this plant. Alkaloids were deemed as its characteristic constituents. This review summarized the biological activities and chemical constituents of L. glutinosa for the first time. Based on these collected information, we pointed out that little information was found related to the potential mechanism of the biological effects. Moreover, there were few reports regarding the biological evaluation of the isolated compounds. As the development of new technique in isolation and biochemistry, close attention should be paid to biological evaluation and potential mechanism.

In vivo analgesic, antipyretic, and anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic extract from Litsea glutinosaleaves

BACKGROUND: The study was conducted to evaluate the in vitro thrombolytic activity, and in vivo analgesic, anti-inflammatory and antipyretic potentials of different hydrocarbon soluble extracts of Litsea glutinosaleaves for the first time widely used in the folkloric treatments in Bangladesh. This work aimed to create new insights on the fundamental mechanisms of the plant extracts involved in these activities. RESULTS: In thrombolytic activity assay, a significant clot disruption was observed at dose of 1 mg/mL for each of the extracts (volume 100 µL) when compared to the standard drug streptokinase. The n-hexane, ethyl acetate, chloroform, and crude methanolic extracts showed 32.23 ± 0.26, 37.67 ± 1.31, 43.13 ± 0.85, and 46.78 ± 0.9% clot lysis, respectively, whereas the positive control streptokinase showed 93.35 ± 0.35% disruption at the dose of 30,000 I.U. In hot plate method, the highest pain inhibitory activity was found at a dose of 500 mg/kg of crude extract (15.54 ± 0.37 sec) which differed significantly (P <0.01 and P <0.001) with that of the standard drug ketorolac (16.38 ± 0.27 sec). In acetic acid induced writhing test, the crude methanolic extract showed significant (P <0.01 and P <0.001) analgesic potential at doses 250 and 500 mg/kg body weight (45.98 and 56.32% inhibition, respectively), where ketorolac showed 64.36% inhibition. In anti-inflammatory activity test, the crude methanolic extract showed significant (P <0.001) potential at doses 250 and 500 mg/kg body weight (1.51 ± 0.04 and 1.47 ± 0.03 mm paw edema, respectively), where ketorolac showed 1.64 ± 0.05 mm edema after 3 h of carrageenan injection. In antipyretic activity assay, the crude extract showed notable reduction in body temperature (32.78 ± 0.46°C) at dose of 500 mg/kg-body weight, when the standard (at dose 150 mg/kg-body weight) exerted 33.32 ± 0.67°C temperature after 3 h of administration. CONCLUSIONS: Our results yield that the crude hydroalcoholic extract has better effects than the other in all trials. In the context, it can be said that the leaves of L. glutinosa possess remarkable pharmacological effects, and justify its traditional use as analgesic, antipyretic, anti-inflammatory, and thrombolytic agent.

Extraction and phytochemical evaluation of litseaglutinosa bark methanolic extract.

The present investigation deals with the extraction of bark powder of Litsea glutinosa and its preliminary phytochemical screening. The bark powder was subjected to methanolic extraction and further explored for its phytochemical constituents using TLC and GC MS. Preliminary phytochemical screening reveals the presence of alkaloids, flavonoides, glycosides, phenols, tannins and saponins. The extract was further subjected to separation using TLC and fractions were evaluated using GC MS. GC MS analysis of the total methanolic extract showed the presence of Oliec acid, tricosene, erucic acid, tetra decanoic acid, pyrrolidinone, piperidine, eicosanoic acid like major phytochemicals. Alkaloid fraction was found to be rich in therapeutically potential compounds like Eicosane, Pieprizine, pyridine, thio-coumarin, tetrahydroisoquinoline. Apart from this various Androstane, Androsta-trione, pregnene like phytoestrogens were also observed in this plant, justifying its aphrodisiac and osteoprotective effect. TLC of various subfractions of alkaloids revealed that this plant is rich in variety of potential therapeutic phytochemicals, hence justifying its ethnomedical usage.